Transcription factor E3, a major regulator of mast cell-mediated allergic response

Yagil, Zohar and Hadad Erlich, Tal and Ofir-Birin, Yifat and Tshori, Sagi and Kay, Gillian and Yekhtin, Zanna and Fisher, David E. and Cheng, Chang and Wong, W. S. Fred and Hartmann, Karin and Razin, Ehud and Nechushtan, Hovav. (2012) Transcription factor E3, a major regulator of mast cell-mediated allergic response. The Journal of Allergy & Clinical Immunology, 129 (5). pp. 1357-1366.e5.

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Official URL: https://edoc.unibas.ch/70807/

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Microphthalmia transcription factor, an MiT transcription family member closely related to transcription factor E3 (TFE3), is essential for mast cell development and survival. TFE3 was previously reported to play a role in the functions of B and T cells; however, its role in mast cells has not yet been explored.; We sought to explore the role played by TFE3 in mast cell function.; Mast cell numbers were evaluated by using toluidine blue staining. FACS analysis was used to determine percentages of Kit and FcεRI double-positive cells in the peritoneum of wild-type (WT) and TFE3 knockout (TFE3(-/-)) mice. Cytokine and inflammatory mediator secretion were measured in immunologically activated cultured mast cells derived from either knockout or WT mice. In vivo plasma histamine levels were measured after immunologic triggering of these mice.; No significant differences in mast cell numbers between WT and TFE3(-/-) mice were observed in the peritoneum, lung, and skin. However, TFE3(-/-) mice showed a marked decrease in the number of Kit(+) and FcεRI(+) peritoneal and cultured mast cells. Surface expression levels of FcεRI in TFE3(-/-) peritoneal mast cells was significantly lower than in control cells. Cultured mast cells derived from TFE3(-/-) mice showed a marked decrease in degranulation and mediator secretion. In vivo experiments showed that the level of plasma histamine in TFE3(-/-) mice after an allergic trigger was substantially less than that seen in WT mice.; TFE3 is a novel regulator of mast cell functions and as such could emerge as a new target for the manipulation of allergic diseases.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Allergy and Immunity (Hartmann)
UniBasel Contributors:Hartmann, Karin
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:10 Nov 2020 15:45
Deposited On:10 Nov 2020 15:45

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