IκB kinase 2 is essential for IgE-induced mast cell de novo cytokine production but not for degranulation

Peschke, Katrin and Weitzmann, Anke and Heger, Klaus and Behrendt, Rayk and Schubert, Nadja and Scholten, Julia and Voehringer, David and Hartmann, Karin and Dudeck, Anne and Schmidt-Supprian, Marc and Roers, Axel. (2014) IκB kinase 2 is essential for IgE-induced mast cell de novo cytokine production but not for degranulation. Cell Reports, 8 (5). pp. 1300-1307.

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Official URL: https://edoc.unibas.ch/70769/

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The immunoglobulin E (IgE)-mediated mast cell (MC) response is central to the pathogenesis of type I allergy and asthma. IκB kinase 2 (IKK2) was reported to couple IgE-induced signals to MC degranulation by phosphorylating the SNARE protein SNAP23. We investigated MC responses in mice with MC-specific inactivation of IKK2 or NF-κB essential modulator (NEMO), or animals with MC-specific expression of a mutant, constitutively active IKK2. We show that the IgE-induced late-phase cytokine response is reduced in mice lacking IKK2 or NEMO in MCs. However, anaphylactic in vivo responses of these animals are not different from those of control mice, and in vitro IKK2-deficient MCs readily phosphorylate SNAP23 and degranulate similarly to control cells in response to allergen or calcium ionophore. Constitutive overactivation of the NF-κB pathway has only slight effects on allergen-triggered MC responses. Thus, IKK2 is dispensable for MC degranulation, and the important question how IgE-induced signals trigger MC vesicle fusion remains open.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Allergy and Immunity (Hartmann)
UniBasel Contributors:Hartmann, Karin
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Cell Press
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:01 Jun 2020 11:32
Deposited On:01 Jun 2020 11:32

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