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Regulated nuclear accumulation of a histone methyltransferase times the onset of heterochromatin formation in; C. elegans; embryos

Mutlu, Beste and Chen, Huei-Mei and Moresco, James J. and Orelo, Barbara D. and Yang, Bing and Gaspar, John M. and Keppler-Ross, Sabine and Yates, John R. and Hall, David H. and Maine, Eleanor M. and Mango, Susan E.. (2018) Regulated nuclear accumulation of a histone methyltransferase times the onset of heterochromatin formation in; C. elegans; embryos. Science advances, 4 (8). eaat6224.

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Official URL: https://edoc.unibas.ch/70544/

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Abstract

Heterochromatin formation during early embryogenesis is timed precisely, but how this process is regulated remains elusive. We report the discovery of a histone methyltransferase complex whose nuclear accumulation and activation establish the onset of heterochromatin formation in; Caenorhabditis elegans; embryos. We find that the inception of heterochromatin generation coincides with the accumulation of the histone H3 lysine 9 (H3K9) methyltransferase MET-2 (SETDB) into nuclear hubs. The absence of MET-2 results in delayed and disturbed heterochromatin formation, whereas accelerated nuclear localization of the methyltransferase leads to precocious H3K9 methylation. We identify two factors that bind to and function with MET-2: LIN-65, which resembles activating transcription factor 7-interacting protein (ATF7IP) and localizes MET-2 into nuclear hubs, and ARLE-14, which is orthologous to adenosine 5'-diphosphate-ribosylation factor-like 14 effector protein (ARL14EP) and promotes stable association of MET-2 with chromatin. These data reveal that nuclear accumulation of MET-2 in conjunction with LIN-65 and ARLE-14 regulates timing of heterochromatin domains during embryogenesis.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Cell and Developmental Biology (Mango)
UniBasel Contributors:Mango, Susan Elizabeth
Item Type:Article, refereed
Article Subtype:Research Article
ISSN:2375-2548
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:09 Apr 2020 15:33
Deposited On:09 Apr 2020 15:33

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