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Cryo-EM structures of the pore-forming A subunit from the Yersinia entomophaga ABC toxin

Piper, Sarah J. and Brillault, Lou and Rothnagel, Rosalba and Croll, Tristan I. and Box, Joseph K. and Chassagnon, Irene and Scherer, Sebastian and Goldie, Kenneth N. and Jones, Sandra A. and Schepers, Femke and Hartley-Tassell, Lauren and Ve, Thomas and Busby, Jason N. and Dalziel, Julie E. and Lott, J. Shaun and Hankamer, Ben and Stahlberg, Henning and Hurst, Mark R. H. and Landsberg, Michael J.. (2019) Cryo-EM structures of the pore-forming A subunit from the Yersinia entomophaga ABC toxin. Nature Communications, 10 (1). p. 1952.

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Official URL: https://edoc.unibas.ch/70377/

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Abstract

ABC toxins are pore-forming virulence factors produced by pathogenic bacteria. YenTcA is the pore-forming and membrane binding A subunit of the ABC toxin YenTc, produced by the insect pathogen Yersinia entomophaga. Here we present cryo-EM structures of YenTcA, purified from the native source. The soluble pre-pore structure, determined at an average resolution of 4.4 Å, reveals a pentameric assembly that in contrast to other characterised ABC toxins is formed by two TcA-like proteins (YenA1 and YenA2) and decorated by two endochitinases (Chi1 and Chi2). We also identify conformational changes that accompany membrane pore formation by visualising YenTcA inserted into liposomes. A clear outward rotation of the Chi1 subunits allows for access of the protruding translocation pore to the membrane. Our results highlight structural and functional diversity within the ABC toxin subfamily, explaining how different ABC toxins are capable of recognising diverse hosts.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Stahlberg)
05 Faculty of Science
UniBasel Contributors:Stahlberg, Henning and Goldie, Kenneth N.
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Publishing Group
e-ISSN:2041-1723
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:05 Oct 2020 06:50
Deposited On:14 Aug 2020 14:33

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