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Detection of dicistroviruses RNA in blood of febrile Tanzanian children

Cordey, Samuel and Laubscher, Florian and Hartley, Mary-Anne and Junier, Thomas and Pérez-Rodriguez, Francisco J. and Keitel, Kristina and Vieille, Gael and Samaka, Josephine and Mlaganile, Tarsis and Kagoro, Frank and Boillat-Blanco, Noémie and Mbarack, Zainab and Docquier, Mylène and Brito, Francisco and Eibach, Daniel and May, Jürgen and Sothmann, Peter and Aldrich, Cassandra and Lusingu, John and Tapparel, Caroline and D'Acremont, Valérie and Kaiser, Laurent. (2019) Detection of dicistroviruses RNA in blood of febrile Tanzanian children. Emerging microbes and infections, 8 (1). pp. 613-623.

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Abstract

Fever is the leading cause of paediatric outpatient consultations in Sub-Saharan Africa. Although most are suspected to be of viral origin, a putative causative pathogen is not identified in over a quarter of these febrile episodes. Using a de novo assembly sequencing approach, we report the detection (15.4%) of dicistroviruses (DicV) RNA in sera collected from 692 febrile Tanzanian children. In contrast, DicV RNA was only detected in 1/77 (1.3%) plasma samples from febrile Tanzanian adults, suggesting that children could represent the primary susceptible population. Estimated viral load by specific quantitative real-time RT-PCR assay ranged from < 1.32E3 to 1.44E7 viral RNA copies/mL serum. Three DicV full-length genomes were obtained, and a phylogenetic analyse on the capsid region showed the presence of two clusters representing tentative novel genus. Although DicV-positive cases were detected throughout the year, a significantly higher positivity rate was observed during the rainy season. This study reveals that novel DicV RNA is frequently detected in the blood of Tanzanian children, paving the way for further investigations to determine if DicV possibly represent a new agent in humans.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Epidemiology and Public Health (EPH) > Health Interventions > Clinical Epidemiology (Genton)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Medicine > Clinical Research (Reither)
UniBasel Contributors:Keitel, Kristina and D'Acremont, Valérie
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Publishing Group
ISSN:2222-1751
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:25 Apr 2019 08:45
Deposited On:25 Apr 2019 08:45

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