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Insights into the genetic diversity of Mycobacterium tuberculosis in Tanzania

Rutaihwa, Liliana K. and Sasamalo, Mohamed and Jaleco, Aladino and Hella, Jerry and Kingazi, Ally and Kamwela, Lujeko and Kingalu, Amri and Malewo, Bryceson and Shirima, Raymond and Doetsch, Anna and Feldmann, Julia and Reinhard, Miriam and Borrell, Sonia and Brites, Daniela and Reither, Klaus and Doulla, Basra and Fenner, Lukas and Gagneux, Sebastien. (2019) Insights into the genetic diversity of Mycobacterium tuberculosis in Tanzania. PLoS ONE, 14 (4). e0206334.

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Official URL: https://edoc.unibas.ch/70271/

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Abstract

Human tuberculosis (TB) is caused by seven phylogenetic lineages of the Mycobacterium tuberculosis complex (MTBC), Lineage 1-7. Recent advances in rapid genotyping of MTBC based on single nucleotide polymorphisms (SNP), allow for phylogenetically robust strain classification, paving the way for defining genotype-phenotype relationships in clinical settings. Such studies have revealed that, in addition to host and environmental factors, strain variation in the MTBC influences the outcome of TB infection and disease. In Tanzania, such molecular epidemiological studies of TB however are scarce in spite of a high TB burden.; Here we used SNP-typing to characterize a nationwide collection of 2,039 MTBC clinical isolates representative of 1.6% of all new and retreatment TB cases notified in Tanzania during 2012 and 2013. Four lineages, namely Lineage 1-4 were identified within the study population. The distribution and frequency of these lineages varied across regions but overall, Lineage 4 was the most frequent (n = 866, 42.5%), followed by Lineage 3 (n = 681, 33.4%) and 1 (n = 336, 16.5%), with Lineage 2 being the least frequent (n = 92, 4.5%). We found Lineage 2 to be independently associated with female sex (adjusted odds ratio [aOR] 2.14; 95% confidence interval [95% CI] 1.31 - 3.50, p = 0.002) and retreatment cases (aOR 1.67; 95% CI 0.95 - 2.84, p = 0. 065) in the study population. We found no associations between MTBC lineage and patient age or HIV status. Our sublineage typing based on spacer oligotyping on a subset of Lineage 1, 3 and 4 strains revealed the presence of mainly EAI, CAS and LAM families. Finally, we detected low levels of multidrug resistant isolates among a subset of 144 retreatment cases.; This study provides novel insights into the MTBC lineages and the possible influence of pathogen-related factors on the TB epidemic in Tanzania.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology > Tuberculosis Research (Gagneux)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Medicine > Clinical Research (Reither)
UniBasel Contributors:Rutaihwa, Liliana and Hella, Jerry and Jaleco, Aladino Junjun II and Kamwela, Lujeko and Dötsch, Anna and Feldmann, Julia and Reinhard, Miriam and Borrell Farnov, Sonia and Brites, Daniela and Reither, Klaus and Fenner, Lukas and Gagneux, Sebastien
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Public Library of Science
e-ISSN:1932-6203
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:23 Apr 2019 09:36
Deposited On:23 Apr 2019 09:36

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