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Quantification of viable bacterial load in artificial sputum spiked with Mycobacterium tuberculosis

Friedrich, Sven O. and Kolwijck, Eva and Karinja, Miriam N. and van der Merwe, Lize and Diacon, Andreas H.. (2019) Quantification of viable bacterial load in artificial sputum spiked with Mycobacterium tuberculosis. Tuberculosis, 115. pp. 140-145.

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Official URL: https://edoc.unibas.ch/70155/

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Abstract

Artificial sputum spiked with Mycobacterium tuberculosis could serve for validation of procedures that determine viable mycobacterial load.; Artificial sputum specimens prepared in-house were spiked with low, medium or high concentrations of Mycobacterium tuberculosis H37Rv stock solution. In a first series, a single technologist processed two batches of specimens daily with high load that were stored refrigerated or at room temperature for up to 8 days. In a second series, nine different technologists processed freshly made batches of specimens with low, medium or high loads. We recorded time to positivity (TTP) in duplicate liquid cultures made from each specimen.; Specimens were well grouped around the mean TTP (hours; standard deviation) of low: 271.7 (25.9), medium: 233.5 (16.3), and two batches of high load: 186.9 (12.3) and 191.8 (9.0), respectively. A variance component model that included load, storage temperature, days of storage until processing, batch of specimens made, sample ID and technologist ID as random effects in a linear mixed-effects model identified only load, technologist and residual as significant contributors to overall TTP variance.; Artificial sputum specimens with reproducible and stable viable mycobacterial loads can be made that could serve for training and validation purposes.
Faculties and Departments:03 Faculty of Medicine > Departement Public Health > Sozial- und Präventivmedizin > Malaria Vaccines (Tanner)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Epidemiology and Public Health (EPH) > Health Interventions > Malaria Vaccines (Tanner)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
UniBasel Contributors:Karinja, Miriam
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Elsevier
ISSN:1472-9792
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:11 Apr 2019 12:50
Deposited On:11 Apr 2019 12:50

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