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Contribution of RNA Degradation to Intrinsic and Extrinsic Noise in Gene Expression

Baudrimont, Antoine and Jaquet, Vincent and Wallerich, Sandrine and Voegeli, Sylvia and Becskei, Attila. (2019) Contribution of RNA Degradation to Intrinsic and Extrinsic Noise in Gene Expression. Cell reports, 26 (13). 3752-3761.e5.

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Official URL: https://edoc.unibas.ch/70127/

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Abstract

Genetically identical cells contain variable numbers of molecules, even if the cells share the same environment. This stochastic variability is prominent when molecules have low abundance, which is the case for mRNA noise. Most studies focused on how transcription affects mRNA noise, and little is known about the role of RNA degradation. To discriminate the fluctuations in these processes during the decay of a pair of reporter mRNAs, we quantified the uncorrelated intrinsic and the correlated extrinsic noise using single-molecule RNA FISH. Intrinsic noise converges to the Poisson level during the decay. mRNAs that have a short half-life are more susceptible to extrinsic noise than stable mRNAs. However, the Xrn1 exonuclease and the NMD pathways, which degrade mRNAs rapidly, were found to have lower fluctuation, which mitigates the noise of the short-lived mRNAs. This permits low variability across the entire range of mRNA half-lives.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Computational & Systems Biology > Synthetic Microbiology (Becskei)
UniBasel Contributors:Becskei, Attila and Baudrimont, Antoine and Jaquet, Vincent and Wallerich, Sandrine and Vögeli, Sylvia
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Elsevier
ISSN: to Intrinsic and Extrinsic Noise in Gene Expression
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:05 Apr 2019 13:05
Deposited On:05 Apr 2019 13:04

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