Conformations and cryo-force spectroscopy of spray-deposited single-strand DNA on gold

Pawlak, Rémy and Vilhena, J. G. and Hinaut, Antoine and Meier, Tobias and Glatzel, Thilo and Baratoff, Alexis and Gnecco, Enrico and Pérez, Rubén and Meyer, Ernst. (2019) Conformations and cryo-force spectroscopy of spray-deposited single-strand DNA on gold. Nature Communications, 10 (1). p. 685.

[img] PDF - Published Version
Available under License CC BY (Attribution).


Official URL: https://edoc.unibas.ch/70125/

Downloads: Statistics Overview


Cryo-electron microscopy can determine the structure of biological matter in vitrified liquids. However, structure alone is insufficient to understand the function of native and engineered biomolecules. So far, their mechanical properties have mainly been probed at room temperature using tens of pico-newton forces with a resolution limited by thermal fluctuations. Here we combine force spectroscopy and computer simulations in cryogenic conditions to quantify adhesion and intra-molecular properties of spray-deposited single-strand DNA oligomers on Au(111). Sub-nanometer resolution images reveal folding conformations confirmed by simulations. Lifting shows a decay of the measured stiffness with sharp dips every 0.2-0.3âEuro‰nm associated with the sequential peeling and detachment of single nucleotides. A stiffness of 30-35âEuro‰NâEuro‰mâˆ'1 per stretched repeat unit is deduced in the nano-newton range. This combined study suggests how to better control cryo-force spectroscopy of adsorbed heterogeneous (bio)polymer and to potentially enable single-base recognition in DNA strands only few nanometers long.
Faculties and Departments:05 Faculty of Science > Departement Physik > Physik > Nanomechanik (Meyer)
UniBasel Contributors:Glatzel, Thilo and Pawlak, Rémy and Vilhena Albuquerque D'Orey, José Guilherme and Hinaut, Antoine and Meier, Tobias and Baratoff, Alexis and Meyer, Ernst
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Publishing Group
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
edoc DOI:
Last Modified:06 Apr 2022 14:12
Deposited On:05 Apr 2019 12:46

Repository Staff Only: item control page