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A surface-induced asymmetric program promotes tissue colonization by Pseudomonas aeruginosa

Laventie, Benoît-Joseph and Sangermani, Matteo and Estermann, Fabienne and Manfredi, Pablo and Planes, Rémi and Hug, Isabelle and Jaeger, Tina and Meunier, Etienne and Broz, Petr and Jenal, Urs. (2019) A surface-induced asymmetric program promotes tissue colonization by Pseudomonas aeruginosa. Cell host & microbe, 25 (1). pp. 140-152.E6.

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Official URL: https://edoc.unibas.ch/69862/

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Abstract

The opportunistic human pathogen Pseudomonas aeruginosa effectively colonizes host epithelia using pili as primary adhesins. Here we uncover a surface-specific asymmetric virulence program that greatly enhances P. aeruginosa host colonization. We show that when P. aeruginosa encounters surfaces, the concentration of the second messenger c-di-GMP increases within a few seconds. This leads to surface adherence and virulence induction by stimulating pili assembly through the activation of the c-di-GMP receptor FimW. Surface attached bacteria divide asymmetrically generating a piliated, surface committed progeny and a flagellated, motile offspring that leaves the surface to colonize distant sites. Cell differentiation is driven by a phosphodiesterase that asymmetrically positions to the flagellated pole thereby maintaining c-di-GMP levels low in the motile offspring. Infection experiments demonstrate that cellular asymmetry strongly boosts infection spread and tissue damage. Thus, P. aeruginosa promotes surface colonization and infection transmission through a cooperative virulence program that we termed Touch-Seed-and-Go.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Molecular Microbiology (Jenal)
05 Faculty of Science > Departement Biozentrum > Growth & Development > Molecular Microbiology (Jenal)
05 Faculty of Science > Departement Biozentrum > Infection Biology
UniBasel Contributors:Jenal, Urs and Laventie, Benoit-Joseph and Manfredi, Pablo and Estermann, Fabienne and Sangermani, Matteo and Hug, Isabelle and Jaeger, Tina
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Elsevier
ISSN:1931-3128
e-ISSN:1934-6069
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:07 Aug 2020 13:16
Deposited On:07 Aug 2020 13:16

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