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Isothermal microcalorimetry - a quantitative method to monitor Trypanosoma congolense growth and growth inhibition by trypanocidal drugs in real time

Gysin, M. and Braissant, O. and Gillingwater, K. and Brun, R. and Mäser, P. and Wenzler, T.. (2018) Isothermal microcalorimetry - a quantitative method to monitor Trypanosoma congolense growth and growth inhibition by trypanocidal drugs in real time. International journal for parasitology. Drugs and drug resistance, 8 (2). pp. 159-164.

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Abstract

Trypanosoma congolense is a protozoan parasite that is transmitted by tsetse flies, causing African Animal Trypanosomiasis, also known as Nagana, in sub-Saharan Africa. Nagana is a fatal disease of livestock that causes severe economic losses. Two drugs are available, diminazene and isometamidium, yet successful treatment is jeopardized by drug resistant T. congolense. Isothermal microcalorimetry is a highly sensitive tool that can be used to study growth of the extracellular T. congolense parasites or to study parasite growth inhibition after the addition of antitrypanosomal drugs. Time of drug action and time to kill can be quantified in a simple way by real time heat flow measurements. We established a robust protocol for the microcalorimetric studies of T. congolense and developed mathematical computations in R to calculate different parameters related to growth and the kinetics of drug action. We demonstrate the feasibility and benefit of the method exemplary with the two standard drugs, diminazene aceturate and isometamidium chloride. The method and the mathematical approach can be translated to study other pathogenic or non-pathogenic cells if they are metabolically active and grow under axenic conditions.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology > Parasite Chemotherapy (Mäser)
UniBasel Contributors:Gillingwater, Kirsten and Brun, Reto and Mäser, Pascal and Wenzler, Tanja
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Elsevier
ISSN:2211-3207
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:04 Mar 2019 10:52
Deposited On:04 Mar 2019 10:52

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