Combining Voltage and Calcium Imaging from Neuronal Dendrites

Canepari, Marco and Vogt, Kaspar and Zecevic, Dejan. (2008) Combining Voltage and Calcium Imaging from Neuronal Dendrites. Cellular and molecular neurobiology, Vol. 28, No. 8 , 1079-1093.

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Official URL: http://edoc.unibas.ch/dok/A5259778

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The ability to monitor membrane potential (V (m)) and calcium (Ca(2+)) transients at multiple locations on the same neuron can facilitate further progress in our understanding of neuronal function. Here we describe a method to combine V (m) and Ca(2+) imaging using styryl voltage sensitive dyes and Fura type UV-excitable Ca(2+) indicators. In all cases V (m) optical signals are linear with membrane potential changes, but the calibration of optical signals on an absolute scale is presently possible only in some neurons. The interpretation of Ca(2+) optical signals depends on the indicator Ca(2+) buffering capacity relative to the cell endogenous buffering capacity. In hippocampal CA1 pyramidal neurons, loaded with JPW-3028 and 300 muM Bis-Fura-2, V (m) optical signals cannot be calibrated and the physiological Ca(2+) dynamics are compromised by the presence of the indicator. Nevertheless, at each individual site, relative changes in V (m) and Ca(2+) fluorescence signals under different conditions can provide meaningful new information on local dendritic integration. In cerebellar Purkinje neurons, loaded with JPW-1114 and 1 mM Fura-FF, V (m) optical signals can be calibrated in terms of mV and Ca(2+) optical signals quantitatively reveal the physiological changes in free Ca(2+). Using these two examples, the method is explained in detail.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Pharmacology/Neurobiology (Vogt)
03 Faculty of Medicine > Departement Biomedizin > Former Units at DBM > Pharmakologie (Vogt)
UniBasel Contributors:Vogt, Kaspar
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Plenum Press
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:21 Jun 2013 12:25
Deposited On:22 Mar 2012 13:36

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