Scale-invariant patterning by size-dependent inhibition of Nodal signalling

Almuedo-Castillo, María and Bläßle, Alexander and Mörsdorf, David and Marcon, Luciano and Soh, Gary H. and Rogers, Katherine W. and Schier, Alexander F. and Müller, Patrick. (2018) Scale-invariant patterning by size-dependent inhibition of Nodal signalling. Nature cell biology, 20 (9). pp. 1032-1042.

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Official URL: https://edoc.unibas.ch/69126/

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Individuals can vary substantially in size, but the proportions of their body plans are often maintained. We generated smaller zebrafish by removing 30% of their cells at the blastula stages and found that these embryos developed into normally patterned individuals. Strikingly, the proportions of all germ layers adjusted to the new embryo size within 2 hours after cell removal. As Nodal-Lefty signalling controls germ-layer patterning, we performed a computational screen for scale-invariant models of this activator-inhibitor system. This analysis predicted that the concentration of the highly diffusive inhibitor Lefty increases in smaller embryos, leading to a decreased Nodal activity range and contracted germ-layer dimensions. In vivo studies confirmed that Lefty concentration increased in smaller embryos, and embryos with reduced Lefty levels or with diffusion-hindered Lefty failed to scale their tissue proportions. These results reveal that size-dependent inhibition of Nodal signalling allows scale-invariant patterning.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Cell and Developmental Biology (Schier)
UniBasel Contributors:Schier, Alexander
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:10 Apr 2020 17:07
Deposited On:10 Apr 2020 17:07

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