Opitz, Christian and Ahrné, Erik and Goldie, Kenneth N. and Schmidt, Alexander and Grzesiek, Stephan. (2019) Deuterium induces a distinctive Escherichia coli proteome that correlates with the reduction in growth rate. Journal of Biological Chemistry, 294 (7). pp. 2279-2292.
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Official URL: https://edoc.unibas.ch/68528/
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Abstract
Substitution of protium (H) for deuterium (D) strongly affects biological systems. Whereas higher eukaryotes such as plants and mammals hardly survive a deuterium content of >30%, many microorganisms can grow on fully deuterated media, albeit at reduced rates. Very little is known about how the H/D replacement influences life at the systems level. Here, we used MS-based analysis to follow the adaptation of a large part of the; Escherichia coli; proteome from growth on a protonated full medium, over a protonated minimal medium, to a completely deuterated minimal medium. We could quantify >1800 proteins under all conditions, several 100 of which exhibited strong regulation during both adaptation processes. The adaptation to minimal medium strongly up-regulated amino acid synthesis and sugar metabolism and down-regulated translational proteins on average by 9%, concomitant with a reduction in growth rate from 1.8 to 0.67 h; -1; In contrast, deuteration caused a very wide proteomic response over many cell functional categories, together with an additional down-regulation of the translational proteins by 5%. The latter coincided with a further reduction in growth rate to 0.37 h; -1; , revealing a clear linear correlation between growth rate and abundance of translational proteins. No significant morphological effects are observed under light and electron microscopies. Across all protein categories, about 80% of the proteins up-regulated under deuteration are enzymes with hydrogen transfer functions. Thus, the H/D kinetic isotope effect appears as the major limiting factor of cellular functions under deuteration.
Faculties and Departments: | 05 Faculty of Science 05 Faculty of Science > Departement Biozentrum 05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Grzesiek) |
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UniBasel Contributors: | Grzesiek, Stephan and Ahrné, Erik and Goldie, Kenneth N. and Schmidt, Alexander |
Item Type: | Article, refereed |
Article Subtype: | Research Article |
Publisher: | American Society for Biochemistry and Molecular Biology |
ISSN: | 0021-9258 |
e-ISSN: | 1083-351X |
Note: | Publication type according to Uni Basel Research Database: Journal article |
Language: | English |
Language: | English |
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Last Modified: | 26 Oct 2023 15:40 |
Deposited On: | 10 Jan 2020 08:05 |
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