Detecting Selective Protein Binding inside Plasmonic Nanopores: Towards a Mimic of the Nuclear Pore Complex

Malekian, Bita and Schoch, Rafael L. and Robson, Timothy and Ferrand-Drake del Castillo, Gustav and Xiong, Kunli and Emilsson, Gustav and Kapinos, Larisa E. and Lim, Roderick Y. H. and Dahlin, Andreas B.. (2018) Detecting Selective Protein Binding inside Plasmonic Nanopores: Towards a Mimic of the Nuclear Pore Complex. Frontiers in Chemistry, 6. p. 637.

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Official URL: https://edoc.unibas.ch/68203/

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Biosensors based on plasmonic nanostructures offer label-free and real-time monitoring of biomolecular interactions. However, so do many other surface sensitive techniques with equal or better resolution in terms of surface coverage. Yet, plasmonic nanostructures offer unique possibilities to study effects associated with nanoscale geometry. In this work we use plasmonic nanopores with double gold films and detect binding of proteins inside them. By thiol and trietoxysilane chemistry, receptors are selectively positioned on the silicon nitride interior walls. Larger (∼150 nm) nanopores are used detect binding of averaged sized proteins (∼60 kg/mol) with high signal to noise (>100). Further, we fabricate pores that approach the size of the nuclear pore complex (diameter down to 50 nm) and graft disordered phenylalanine-glycine nucleoporin domains to the walls, followed by titration of karyopherinβ1 transport receptors. The interactions are shown to occur with similar affinity as determined by conventional surface plasmon resonance on planar surfaces. Our work illustrates another unique application of plasmonic nanostructures, namely the possibility to mimic the geometry of a biological nanomachine with integrated optical sensing capabilities.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Nanobiology Argovia (Lim)
UniBasel Contributors:Lim, Roderick Y.H. and Schoch, Rafael and Kapinos Schneider, Larisa E. E
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Frontiers Research Foundation
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:03 Aug 2020 15:25
Deposited On:03 Aug 2020 15:25

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