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Three-dimensional cell culture and tissue engineering in a T-CUP (tissue culture under perfusion)

Timmins, Nicholas E. and Scherberich, Arnaud and Früh, Jennifer-Annika and Heberer, Michael and Martin, Ivan and Jakob, Marcel. (2007) Three-dimensional cell culture and tissue engineering in a T-CUP (tissue culture under perfusion). Tissue engineering, Vol. 13, H. 8. pp. 2021-2028.

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Official URL: http://edoc.unibas.ch/dok/A5251316

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Abstract

The aim of this study was to develop and validate a simple and compact bioreactor system for perfusion cell seeding and culture through 3-dimensional porous scaffolds. The developed Tissue Culture Under Perfusion (T-CUP) bioreactor is based on the concept of controlled and confined alternating motion of scaffolds through a cell suspension or culture medium, as opposed to pumping of the fluid through the scaffolds. Via the T-CUP, articular chondrocytes and bone marrow stromal cells could be seeded into porous scaffolds of different compositions and architectures (chronOS, Hyaff-11, and Polyactive) at high efficiency (greater than 75%), uniformity (cells were well distributed throughout the scaffold pores), and viability (greater than 97%). Culture of articular chondrocytes seeded into 4-mm thick Polyactive scaffolds for 2 weeks in the T-CUP resulted in uniform deposition of cartilaginous matrix. Cultivation of freshly isolated human bone marrow nucleated cells seeded into ENGipore ceramic scaffolds for 19 days in the T-CUP resulted in stromal cell-populated constructs capable of inducing ectopic bone formation in nude mice. The T-CUP bioreactor represents an innovative approach to simple, efficient, and reliable 3D cell culture, and could be used either as a model to investigate mechanisms of tissue development or as a graft manufacturing system in the context of regenerative medicine.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Tissue Engineering (Martin)
UniBasel Contributors:Martin, Ivan
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Mary Ann Liebert
ISSN:1076-3279
Note:Publication type according to Uni Basel Research Database: Journal article. This is a copy of an article published in the Tissue engineering © 2007 [copyright Mary Ann Liebert, Inc.]; Tissue engineering is available online at: http://online.liebertpub.com.
Language:English
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Last Modified:31 Dec 2015 10:44
Deposited On:22 Mar 2012 13:36

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