Growth factors for clinical-scale expansion of human articular chondrocytes : Relevance for automated bioreactor systems

Francioli, Silvia-Elena and Martin, Ivan and Sie, Christina-Priska and Hagg, Rupert and Tommasini, Roberto and Candrian, Christian and Heberer, Michael and Barbero, Andrea. (2007) Growth factors for clinical-scale expansion of human articular chondrocytes : Relevance for automated bioreactor systems. Tissue engineering, Vol. 13, H. 6. pp. 1227-1234.

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Official URL: http://edoc.unibas.ch/dok/A5248973

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The expansion of chondrocytes in automated bioreactors for clinical use requires that a relevant number of cells be generated, starting from variable initial seeding densities in one passage and using autologous serum. We investigated whether the growth factor combination transforming growth factor beta 1/fibroblast growth factor 2/platelet-derived growth factor BB (TFP), recently shown to enhance the proliferation capacity of human articular chondrocytes (HACs), allows the efficiency of chondrocyte use to be increased at different seeding densities and percentages of human serum (HS). HACs were seeded at 1,000, 5,000, and 10,000 celIS/cm(2) in medium containing 10 bovine serum or 10,000 cells/cm(2) with 1 chondrogenic capacity of post-expanded HACs was then assessed in pellet cultures. Expansion with TFP allowed a sufficient number of HACs to be obtained in one passage even at the lowest seeding density and HS percentage and variability in cartilage-forming capacity of HACs expanded under the different conditions to be reduced. Instead, larger variations and insufficient yields were found in the absence of TFP. By allowing large numbers of cells to be obtained, starting from a wide range of initial seeding densities and HS percentages, the use of TFP may represent a viable solution for the efficient expansion of HACs and addresses constraints of automated clinical bioreactor systems.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Tissue Engineering (Martin)
UniBasel Contributors:Martin, Ivan
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Mary Ann Liebert
Note:Publication type according to Uni Basel Research Database: Journal article. This is a copy of an article published in the Tissue engineering © 2007 [copyright Mary Ann Liebert, Inc.]; Tissue engineering is available online at: http://online.liebertpub.com.
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Last Modified:31 Dec 2015 10:44
Deposited On:22 Mar 2012 13:36

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