In vitro characterization of immune-related properties of human fetal bone cells for potential tissue engineering applications

Montjovent, Marc-Olivier and Bocelli-Tyndall, Chiara and Scaletta, Corinne and Scherberich, Arnaud and Mark, Silke and Martin, Ivan and Applegate, Lee Ann and Pioletti, Dominique P.. (2009) In vitro characterization of immune-related properties of human fetal bone cells for potential tissue engineering applications. Tissue engineering. Part A, Vol. 15, H. 7. pp. 1523-1532.

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Official URL: http://edoc.unibas.ch/dok/A5251321

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We describe herein some immunological properties of human fetal bone cells recently tested for bone tissue-engineering applications. Adult mesenchymal stem cells (MSCs) and osteoblasts were included in the study for comparison. Surface markers involved in bone metabolism and immune recognition were analyzed using flow cytometry before and after differentiation or treatment with cytokines. Immunomodulatory properties were studied on activated peripheral blood mononuclear cells (PBMCs). The immuno-profile of fetal bone cells was further investigated at the gene expression level. Fetal bone cells and adult MSCs were positive for Stro-1, alkaline phosphatase, CD10, CD44, CD54, and beta2-microglobulin, but human leukocyte antigen (HLA)-I and CD80 were less present than on adult osteoblasts. All cells were negative for HLA-II. Treatment with recombinant human interferon gamma increased the presence of HLA-I in adult cells much more than in fetal cells. In the presence of activated PBMCs, fetal cells had antiproliferative effects, although with patterns not always comparable with those of adult MSCs and osteoblasts. Because of the immunological profile, and with their more-differentiated phenotype than of stem cells, fetal bone cells present an interesting potential for allogeneic cell source in tissue-engineering applications.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Tissue Engineering (Martin)
UniBasel Contributors:Martin, Ivan
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Mary Ann Liebert
Note:Publication type according to Uni Basel Research Database: Journal article. This is a copy of an article published in the Tissue engineering © 2007 [copyright Mary Ann Liebert, Inc.]; Tissue engineering is available online at: http://online.liebertpub.com.
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Last Modified:31 Dec 2015 10:44
Deposited On:22 Mar 2012 13:36

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