Molecular evolution of key metabolic genes during transitions to C-4 and CAM photosynthesis

Goolsby, Eric W. and Moore, Abigail J. and Hancock, Lillian P. and De Vos, Jurriaan M. and Edwards, Erika J.. (2018) Molecular evolution of key metabolic genes during transitions to C-4 and CAM photosynthesis. AMERICAN JOURNAL OF BOTANY, 105 (3). pp. 602-613.

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Official URL: https://edoc.unibas.ch/67312/

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PREMISE OF THE STUDY: Next-generation sequencing facilitates rapid production of well-sampled phylogenies built from very large genetic data sets, which can then be subsequently exploited to examine the molecular evolution of the genes themselves. We present an evolutionary analysis of 83 gene families (19 containing carbon-concentrating mechanism (CCM) genes, 64 containing non-CCM genes) in the portullugo clade (Caryophyllales), a diverse lineage of mostly arid-adapted plants that contains multiple evolutionary origins of all known photosynthesis types in land plants (C-3, C-4, CAM, C-4-CAM, and various intermediates).METHODS: We inferred a phylogeny of 197 individuals from 167 taxa using coalescent-based approaches and individual gene family trees using maximum likelihood. Positive selection analyses were conducted on individual gene family trees with a mixed effects model of evolution (MEME). We devised new indices to compare levels of convergence and prevalence of particular residues between CCM and non-CCM genes and between species with different photosynthetic pathways.KEY RESULTS: Contrary to expectations, there were no significant differences in the levels of positive selection detected in CCM versus non-CCM genes. However, we documented a significantly higher level of convergent amino acid substitutions in CCM genes, especially inC(4) taxa.CONCLUSIONS: Our analyses reveal a new suite of amino acid residues putatively important for C-4 and CAM function. We discuss both the advantages and challenges of using targeted enrichment sequence data for exploratory studies of molecular evolution.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Integrative Biologie > Physiological Plant Ecology (Kahmen)
UniBasel Contributors:de Vos, Jurriaan M.
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:23 Apr 2020 16:10
Deposited On:23 Apr 2020 16:10

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