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Production of isotope-labeled proteins in insect cells for NMR

Franke, Bastian and Opitz, Christian and Isogai, Shin and Grahl, Anne and Delgado, Leonildo and Gossert, Alvar D. and Grzesiek, Stephan. (2018) Production of isotope-labeled proteins in insect cells for NMR. Journal of biomolecular NMR, 71 (3). pp. 173-184.

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Abstract

Baculovirus-infected insect cells have become a powerful tool to express recombinant proteins for structural and functional studies by NMR spectroscopy. This article provides an introduction into the insect cell/baculovirus expression system and its use for the production of recombinant isotope-labeled proteins. We discuss recent advances in inexpensive isotope-labeling methods using labeled algal or yeast extracts as the amino acid source and give examples of advanced NMR applications for proteins, which have become accessible by this eukaryotic expression host.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Grzesiek)
UniBasel Contributors:Grzesiek, Stephan and Franke, Bastian and Opitz, Christian and Isogai, Shin and Grahl, Anne and Cabral Delgado, Leonildo
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Springer
ISSN:0925-2738
e-ISSN:1573-5001
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
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Last Modified:03 Jan 2019 16:53
Deposited On:03 Jan 2019 16:52

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