Franke, Bastian and Opitz, Christian and Isogai, Shin and Grahl, Anne and Delgado, Leonildo and Gossert, Alvar D. and Grzesiek, Stephan. (2018) Production of isotope-labeled proteins in insect cells for NMR. Journal of biomolecular NMR, 71 (3). pp. 173-184.
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Abstract
Baculovirus-infected insect cells have become a powerful tool to express recombinant proteins for structural and functional studies by NMR spectroscopy. This article provides an introduction into the insect cell/baculovirus expression system and its use for the production of recombinant isotope-labeled proteins. We discuss recent advances in inexpensive isotope-labeling methods using labeled algal or yeast extracts as the amino acid source and give examples of advanced NMR applications for proteins, which have become accessible by this eukaryotic expression host.
| Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Grzesiek) |
|---|---|
| UniBasel Contributors: | Grzesiek, Stephan and Franke, Bastian and Opitz, Christian and Isogai, Shin and Grahl, Anne and Cabral Delgado, Leonildo |
| Item Type: | Article, refereed |
| Article Subtype: | Research Article |
| Publisher: | Springer |
| ISSN: | 0925-2738 |
| e-ISSN: | 1573-5001 |
| Note: | Publication type according to Uni Basel Research Database: Journal article |
| Language: | English |
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| Identification Number: |
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| edoc DOI: | |
| Last Modified: | 08 Feb 2020 15:03 |
| Deposited On: | 03 Jan 2019 16:52 |
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