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Production of cell- and tissue-specific monoclonal antibodies for the flatworm Macrostomum sp

Ladurner, Peter and Pfister, Daniela and Seifarth, Christof and Schärer, Lukas and Mahlknecht, Monika and Salvenmoser, Willi and Gerth, Regine and Rieger, Reinhard. (2005) Production of cell- and tissue-specific monoclonal antibodies for the flatworm Macrostomum sp. Histochemistry and cell biology, 123 (1). pp. 89-104.

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Abstract

Abstract Monoclonal antibodies (mABs) against various cell types of the basal free-living flatworm Macrostomum sp. were produced by immunising Balb/c mice with cell suspensions of disintegrated animals. We identified 360 positive supernatants with specific staining of various tissues, cell types, patterns or structures. Here we report immunocytochemical characterisation, histological stainings and isotyping of 11 mABs specific for muscle cells (MMu-1, MMu-2, MMu-3, MMu-4), digestive and prostate glands (MDr-1 and MDr-2, MPr-1), epidermal cells (MEp-1), the ventral nerve cord including neuron clusters (MNv-1), gastrodermal cells (MDa-1) and spermatids (MSp-1). Confocal microscopy, histological techniques, electron microscopy and immunoblotting were applied to demonstrate stainings in juveniles, adults, starved or wellfed animals. Considering the current lack of specific markers the obtained mABs will be particularly helpful studying embryonic and postembryonic development, pattern formation, cell differentiation, regeneration and reproductive allocation in Macrostomum sp., and possibly other basal flatworms. The small size, ease of culturing, short generation time, transparency and the basal phylogenetic position specify Macrostomum sp. as a suitable model organism for comparative analyses within Platyhelminthes and to Drosophila and C. elegans.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Zoologie > Evolutionary Biology (Schärer)
UniBasel Contributors:Schärer, Lukas
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Springer International
ISSN:0301-5564
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:20 Sep 2017 06:26
Deposited On:22 Mar 2012 13:35

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