Gating Protein Transport in Solid State Nanopores by Single Molecule Recognition

Emilsson, Gustav and Sakiyama, Yusuke and Malekian, Bita and Xiong, Kunli and Adali-Kaya, Zeynep and Lim, Roderick Y. H. and Dahlin, Andreas B.. (2018) Gating Protein Transport in Solid State Nanopores by Single Molecule Recognition. ACS Central Science, 4 (8). pp. 1007-1014.

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Official URL: https://edoc.unibas.ch/65576/

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Control of molecular translocation through nanoscale apertures is of great interest for DNA sequencing, biomolecular filters, and new platforms for single molecule analysis. However, methods for controlling the permeability of nanopores are very limited. Here, we show how nanopores functionalized with poly(ethylene glycol) brushes, which fully prevent protein translocation, can be reversibly gated to an “open” state by binding of single IgG antibodies that disrupt the macromolecular barrier. On the basis of surface plasmon resonance data we propose a two-state model describing the antibody–polymer interaction kinetics. Reversibly (weakly) bound antibodies decrease the protein exclusion height while irreversibly (strongly) bound antibodies do not. Our results are further supported by fluorescence readout from pore arrays and high-speed atomic force microscopy on single pores. This type of dynamic barrier control on the nanoscale provides new possibilities for biomolecular separation and analysis.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Nanobiology Argovia (Lim)
UniBasel Contributors:Lim, Roderick Y.H. and Sakiyama, Yusuke
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Chemical Society
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:11 Sep 2020 10:55
Deposited On:11 Sep 2020 10:54

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