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CLIP and cohibin separate rDNA from nucleolar proteins destined for degradation by nucleophagy

Mostofa, Md Golam and Rahman, Muhammad Arifur and Koike, Naoki and Yeasmin, Akter Mst and Islam, Nafisa and Waliullah, Talukdar Muhammad and Hosoyamada, Shun and Shimobayashi, Mitsugu and Kobayashi, Takehiko and Hall, Michael N. and Ushimaru, Takashi. (2018) CLIP and cohibin separate rDNA from nucleolar proteins destined for degradation by nucleophagy. Journal of Cell Biology, 217 (8). p. 2675.

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Abstract

Nutrient starvation or inactivation of target of rapamycin complex 1 (TORC1) in budding yeast induces nucleophagy, a selective autophagy process that preferentially degrades nucleolar components. DNA, including ribosomal DNA (rDNA), is not degraded by nucleophagy, even though rDNA is embedded in the nucleolus. Here, we show that TORC1 inactivation promotes relocalization of nucleolar proteins and rDNA to different sites. Nucleolar proteins move to sites proximal to the nuclear-vacuolar junction (NVJ), where micronucleophagy (or piecemeal microautophagy of the nucleus) occurs, whereas rDNA dissociates from nucleolar proteins and moves to sites distal to NVJs. CLIP and cohibin, which tether rDNA to the inner nuclear membrane, were required for repositioning of nucleolar proteins and rDNA, as well as effective nucleophagic degradation of the nucleolar proteins. Furthermore, micronucleophagy itself was necessary for the repositioning of rDNA and nucleolar proteins. However, rDNA escaped from nucleophagic degradation in CLIP- or cohibin-deficient cells. This study reveals that rDNA-nucleolar protein separation is important for the nucleophagic degradation of nucleolar proteins.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Biochemistry (Hall)
UniBasel Contributors:Hall, Michael N.
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Rockefeller University Press
ISSN:0021-9525
e-ISSN:1540-8140
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:12 Sep 2018 09:28
Deposited On:12 Sep 2018 09:26

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