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Rapid heteromerization and phosphorylation of ligand-activated plant transmembrane receptors and their associated kinase BAK1

Schulze B, Mentzel T. and Jehle AK, Mueller K. and Beeler S, Boller T. and Felix G, Chinchilla D.. (2010) Rapid heteromerization and phosphorylation of ligand-activated plant transmembrane receptors and their associated kinase BAK1. Journal of biological chemistry, Vol. 285 , no. 13. pp. 9444-9451.

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Official URL: http://edoc.unibas.ch/dok/A5262044

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Abstract

In plants leucine rich repeat-receptor kinases (LRR-RKs) located at the plasma membrane play a pivotal function in the perception of extracellular signals. For two of these LRR-RKs, the brassinosteroid receptor BRI1 and the flagellin receptor FLS2, interaction with the LRR-receptor like kinase BAK1 (BRI1 associated receptor kinase 1) was shown to be required for signal transduction. Here we report that FLS2/BAK1 heteromerization occurs almost instantaneously after perception of the ligand, the flagellin-derived peptide flg22. Flg22 can induce formation of a stable FLS2/BAK1 complex in microsomal membrane preparations in vitro and the kinase inhibitor K-252a does not prevent complex formation. A kinase-dead version of BAK1 associates with FLS2 in a flg22-dependent manner but does not restore responsiveness to flg22 in cells of bak1 plants, demonstrating that kinase activity of BAK1 is essential for FLS2 signaling. Furthermore, using in vivo phospho-labeling we are able to detect de novo phosphorylation of both FLS2 and BAK1 within 15 seconds of stimulation with flg22. Similarly, brassinolide induces BAK1 phosphorylation within seconds. Other triggers of plant defense, such as bacterial EF-Tu and the endogenous AtPep1 likewise induce rapid formation of hetero-complexes consisting of de novo phosphorylated BAK1 and proteins representing the ligand-specific binding receptors EFR and PEPR1, respectively. Thus, we propose that several LRR-RKs form tight complexes with BAK1 almost instantaneously after ligand binding, and that the subsequent phosphorylation events are key initial steps in signal transduction.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Botanik > Pflanzenphysiologie Pathogenabwehr (Boller)
UniBasel Contributors:Chinchilla, Delphine and Schulze, Birgit and Mentzel, Tobias and Boller, Thomas
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:American Society of Biological Chemists
ISSN:0021-9258
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:22 Mar 2012 14:23
Deposited On:22 Mar 2012 13:34

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