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Liposome-supported enzymatic peritoneal dialysis

Pratsinis, Anna and Zuercher, Stefanie and Forster, Vincent and Fischer, Eric J. and Luciani, Paola and Leroux, Jean-Christophe. (2017) Liposome-supported enzymatic peritoneal dialysis. Biomaterials, 145. pp. 128-137.

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Official URL: https://edoc.unibas.ch/64197/

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Abstract

Compared to hemodialysis, peritoneal dialysis represents a more straightforward and less invasive alternative, though current solutions are not as effective. Herein, the feasibility of liposome-supported enzymatic peritoneal dialysis (LSEPD) is explored to increase the functionality of peritoneal dialysis for the model indication acute alcohol poisoning. Enzyme-loaded liposomes (E-Liposomes) containing alcohol metabolizing enzymes, alcohol oxidase and catalase, are developed and their in vitro and in vivo performances investigated. The E-Liposomes outperform the free enzymes in stability, overcoming the thermal instability of alcohol oxidase and enhancing the in vitro ethanol elimination, which is further accelerated by hydrogen peroxide, due to the rapid generation of oxygen by catalase. Compared to the free enzymes, the E-Liposomes exhibit reduced systemic exposure and organ distribution. In a rodent ethanol intoxication model, LSEPD enhances ethanol metabolism as evidenced by an increased acetaldehyde production, ethanol's primary metabolite. In conclusion, LSEPD presents an innovative platform to temporarily enhance xenobiotic metabolism, in view of the improved enzyme stability and peritoneal retention.
Faculties and Departments:05 Faculty of Science > Departement Pharmazeutische Wissenschaften > Pharmazie > Pharmazeutische Technologie (Huwyler)
UniBasel Contributors:Pratsinis, Anna
Item Type:Article, refereed
Article Subtype:Research Article
ISSN:1878-5905
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:19 Feb 2019 16:17
Deposited On:19 Feb 2019 16:17

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