Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene

Seuring, Carolin and Ayyer, Kartik and Filippaki, Eleftheria and Barthelmess, Miriam and Longchamp, Jean-Nicolas and Ringler, Philippe and Pardini, Tommaso and Wojtas, David H. and Coleman, Matthew A. and Dörner, Katerina and Fuglerud, Silje and Hammarin, Greger and Habenstein, Birgit and Langkilde, Annette E. and Loquet, Antoine and Meents, Alke and Riek, Roland and Stahlberg, Henning and Boutet, Sébastien and Hunter, Mark S. and Koglin, Jason and Liang, Mengning and Ginn, Helen M. and Millane, Rick P. and Frank, Matthias and Barty, Anton and Chapman, Henry N.. (2018) Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene. Nature communications, 9 (1). p. 1836.

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Official URL: https://edoc.unibas.ch/64181/

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Here we present a new approach to diffraction imaging of amyloid fibrils, combining a free-standing graphene support and single nanofocused X-ray pulses of femtosecond duration from an X-ray free-electron laser. Due to the very low background scattering from the graphene support and mutual alignment of filaments, diffraction from tobacco mosaic virus (TMV) filaments and amyloid protofibrils is obtained to 2.7 Å and 2.4 Å resolution in single diffraction patterns, respectively. Some TMV diffraction patterns exhibit asymmetry that indicates the presence of a limited number of axial rotations in the XFEL focus. Signal-to-noise levels from individual diffraction patterns are enhanced using computational alignment and merging, giving patterns that are superior to those obtainable from synchrotron radiation sources. We anticipate that our approach will be a starting point for further investigations into unsolved structures of filaments and other weakly scattering objects.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Stahlberg)
UniBasel Contributors:Stahlberg, Henning
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:27 Apr 2020 18:16
Deposited On:27 Apr 2020 18:16

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