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FACS-based Isolation of Neural and Glioma Stem Cell Populations from Fresh Human Tissues Utilizing EGF Ligand

Tome-Garcia, Jessica and Doetsch, Fiona and Tsankova, Nadejda M.. (2017) FACS-based Isolation of Neural and Glioma Stem Cell Populations from Fresh Human Tissues Utilizing EGF Ligand. Bio-protocol, 7 (24). p. 23.

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Official URL: https://edoc.unibas.ch/64091/

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Abstract

Direct isolation of human neural and glioma stem cells from fresh tissues permits their biological study without prior culture and may capture novel aspects of their molecular phenotype in their native state. Recently, we demonstrated the ability to prospectively isolate stem cell populations from fresh human germinal matrix and glioblastoma samples, exploiting the ability of cells to bind the Epidermal Growth Factor (EGF) ligand in fluorescence-activated cell sorting (FACS). We demonstrated that FACS-isolated EGF-bound neural and glioblastoma populations encompass the sphere-forming colonies; in vitro; , and are capable of both self-renewal and multilineage differentiation. Here we describe in detail the purification methodology of EGF-bound (; i.e; ., EGFR+) human neural and glioma cells with stem cell properties from fresh postmortem and surgical tissues. The ability to prospectively isolate stem cell populations using native ligand-binding ability opens new doors for understanding both normal and tumor cell biology in uncultured conditions, and is applicable for various downstream molecular sequencing studies at both population and single-cell resolution.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Neurobiology > Stem Cell Biology (Doetsch)
UniBasel Contributors:Doetsch, Fiona
Item Type:Article, refereed
Article Subtype:Research Article
ISSN:2331-8325
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:26 Feb 2019 16:35
Deposited On:26 Feb 2019 16:35

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