Multiple label-free biodetection and quantitative DNA-binding assays on a nanomechanical cantilever array

McKendry, R. and Zhang, J. Y. and Arntz, Y. and Strunz, T. and Hegner, M. and Lang, H. P. and Baller, M. K. and Certa, U. and Meyer, E. and Guntherodt, H. J. and Gerber, C.. (2002) Multiple label-free biodetection and quantitative DNA-binding assays on a nanomechanical cantilever array. Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 15. pp. 9783-9788.

Full text not available from this repository.

Official URL: http://edoc.unibas.ch/dok/A5262136

Downloads: Statistics Overview


We report a microarray of cantilevers to detect multiple unlabeled biomolecules simultaneously at nanomolar concentrations within minutes. Ligand-receptor binding interactions such as DNA hybridization or protein recognition occurring on microfabricated silicon cantilevers generate nanomechanical bending, which is detected optically in situ. Differential measurements including reference cantilevers on an array of eight sensors can sequence-specifically detect unlabeled DNA targets in 80-fold excess of nonmatching DNA as a background and discriminate 3` and 5` overhangs. Our experiments suggest that the nanomechanical motion originates from predominantly steric hindrance effects and depends on the concentration of DNA molecules in solution. We show that cantilever arrays can be used to investigate the thermodynamics of biomolecular interactions mechanically, and we have found that the specificity of the reaction on a cantilever is consistent with solution data. Hence cantilever arrays permit multiple binding assays in parallel and can detect femtomoles of DNA on the cantilever at a DNA concentration in solution of 75 nM.
Faculties and Departments:05 Faculty of Science > Departement Physik > Physik > Nanomechanik (Meyer)
UniBasel Contributors:Meyer, Ernst
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:National Academy of Sciences
Note:Publication type according to Uni Basel Research Database: Journal article
Related URLs:
Identification Number:
Last Modified:22 Mar 2012 14:23
Deposited On:22 Mar 2012 13:34

Repository Staff Only: item control page