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Identification and characterization of the three members of the CLC family of anion transport proteins in Trypanosoma brucei

Steinmann, Michael E. and Schmidt, Remo S. and Macêdo, Juan P. and Kunz Renggli, Christina and Bütikofer, Peter and Rentsch, Doris and Mäser, Pascal and Sigel, Erwin. (2017) Identification and characterization of the three members of the CLC family of anion transport proteins in Trypanosoma brucei. PLoS ONE, 12 (12). e0188219.

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Official URL: https://edoc.unibas.ch/63227/

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Abstract

CLC type anion transport proteins are homo-dimeric or hetero-dimeric with an integrated transport function in each subunit. We have identified and partially characterized three members of this family named TbVCL1, TbVCL2 and TbVCL3 in Trypanosoma brucei. Among the human CLC family members, the T. brucei proteins display highest similarity to CLC-6 and CLC-7. TbVCL1, but not TbVCL2 and TbVCL3 is able to complement growth of a CLC-deficient Saccharomyces cerevisiae mutant. All TbVCL-HA fusion proteins localize intracellulary in procyclic form trypanosomes. TbVCL1 localizes close to the Golgi apparatus and TbVCL2 and TbVCL3 to the endoplasmic reticulum. Upon expression in Xenopus oocytes, all three proteins induce similar outward rectifying chloride ion currents. Currents are sensitive to low concentrations of DIDS, insensitive to the pH in the range 5.4 to 8.4 and larger in nitrate than in chloride medium.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology > Parasite Chemotherapy (Mäser)
UniBasel Contributors:Kunz Renggli, Christina and Mäser, Pascal
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Public Library of Science
e-ISSN:1932-6203
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:31 Aug 2018 06:38
Deposited On:03 Jul 2018 07:41

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