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Evolution of Arabidopsis MIR genes generates novel microRNA classes

Vazquez, Franck and Blevins, Todd and Ailhas, Jérôme and Boller, Thomas and Meins, Frederick. (2008) Evolution of Arabidopsis MIR genes generates novel microRNA classes. Nucleic Acids Research, 36 (20). pp. 6429-6438.

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Official URL: http://edoc.unibas.ch/dok/A5248778

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Abstract

In Arabidopsis, canonical 21-nt miRNAs are generated by Dicer-like (DCL) 1 from hairpin precursors. We have identified a novel class of functional 23- to 25-nt long-miRNAs that is generated independently from the same miRNA precursors by DCL3. Long-miRNAs are developmentally regulated and in some cases have been conserved during evolution implying that they have biological functions. Plant microRNA genes (MIR) have been proposed to evolve by inverted duplication of the target gene. We found that recently evolved MIR genes consistently give rise to long-miRNAs, while ancient MIR genes give rise predominantly to canonical miRNAs. Transcripts from inverted repeats representing evolving proto-MIR genes were processed by DCL3 into long-miRNAs and also by DCL1, DCL2 or DCL4 depending on hairpin stem length to produce different sizes of miRNAs. Our results suggest that evolution of MIR genes is associated with gradual, overlapping changes in DCL usage resulting in specific size classes of miRNAs.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Botanik
UniBasel Contributors:Boller, Thomas and Meins Jr., Frederick and Vazquez, Franck
Item Type:Article
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Oxford University Press
ISSN:0305-1048
e-ISSN:1362-4962
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
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Last Modified:10 Oct 2017 09:07
Deposited On:22 Mar 2012 13:33

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