Real-time tracking, retrieval and gene expression analysis of migrating human T cells

Mehling, M. and Frank, T. and Albayrak, C. and Tay, S.. (2015) Real-time tracking, retrieval and gene expression analysis of migrating human T cells. Lab on a Chip, 15 (5). pp. 1276-1283.

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Official URL: https://edoc.unibas.ch/62356/

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Dynamical analysis of single-cells allows assessment of the extent and role of cell-to-cell variability, however traditional dish-and-pipette techniques have hindered single-cell analysis in quantitative biology. We developed an automated microfluidic cell culture system that generates stable diffusion-based chemokine gradients, where cells can be placed in predetermined positions, monitored via single-cell time-lapse microscopy, and subsequently be retrieved based on their migration speed and directionality for further off-chip gene expression analysis, constituting a powerful platform for multiparameter quantitative studies of single-cell chemotaxis. Using this system we studied CXCL12-directed migration of individual human primary T cells. Spatiotemporally deterministic retrieval of T cell subsets in relation to their migration speed, and subsequent analysis with microfluidic droplet digital-PCR showed that the expression level of CXCR4 - the receptor of CXCL12 - underlies enhanced human T cell chemotaxis.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Translational Neuroimmunology (Mehling)
UniBasel Contributors:Mehling, Matthias
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:19 Nov 2018 17:39
Deposited On:19 Nov 2018 17:39

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