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Miniaturizing EM Sample Preparation: Opportunities, Challenges and "Visual Proteomics"

Arnold, Stefan A. and Müller, Shirley A. and Schmidli, Claudio and Syntychaki, Anastasia and Rima, Luca and Chami, Mohamed and Stahlberg, H. and Goldie, Kenneth N. and Braun, T.. (2018) Miniaturizing EM Sample Preparation: Opportunities, Challenges and "Visual Proteomics". Proteomics. p. 1700176.

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Official URL: https://edoc.unibas.ch/62257/

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Abstract

This review compares and discusses conventional versus miniaturized specimen preparation methods for transmission electron microscopy (EM). The progress brought by direct electron detector cameras, software developments and automation have transformed transmission cryo-electron microscopy (cryo-EM) and made it an invaluable high-resolution structural analysis tool. In contrast, EM specimen preparation has seen very little progress in the last decades and is now one of the main bottlenecks in cryo-EM. Here, we discuss the challenges faced by specimen preparation for single particle EM, highlight current developments, and show the opportunities resulting from the advanced miniaturized and microfluidic sample grid preparation methods described, such as visual proteomics and time-resolved cryo-EM studies. This article is protected by copyright. All rights reserved.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Stahlberg)
UniBasel Contributors:Stahlberg, Henning
Item Type:Article, refereed
Article Subtype:Research Article
ISSN:1615-9861
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:29 Apr 2020 11:33
Deposited On:29 Apr 2020 11:33

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