ALCAM (CD166) is involved in extravasation of monocytes rather than T cells across the blood-brain barrier

Lyck, Ruth and Lécuyer, Marc-André and Abadier, Michael and Wyss, Christof B. and Matti, Christoph and Rosito, Maria and Enzmann, Gaby and Zeis, Thomas and Michel, Laure and García Martín, Ana B. and Sallusto, Federica and Gosselet, Fabien and Deutsch, Urban and Weiner, Joshua A. and Schaeren-Wiemers, Nicole and Prat, Alexandre and Engelhardt, Britta. (2017) ALCAM (CD166) is involved in extravasation of monocytes rather than T cells across the blood-brain barrier. Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 37 (8). pp. 2894-2909.

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Official URL: https://edoc.unibas.ch/62089/

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Activated leukocyte cell adhesion molecule (ALCAM) has been proposed to mediate leukocyte migration across the blood-brain barrier (BBB) in multiple sclerosis or experimental autoimmune encephalomyelitis (EAE). Here, we confirmed vascular ALCAM expression in human brain tissue samples in situ and on two different human in vitro BBB models. Antibody-mediated inhibition of ALCAM reduced diapedesis of human CD4(+) Th1 but not of Th17 cells across the human BBB in vitro. In accordance to human Th1 cells, mouse Th1 cells showed reduced diapedesis across an ALCAM(-/-) in vitro BBB model under static but no longer under flow conditions. In contrast to the limited role of ALCAM in T cell extravasation across the BBB, we found a contribution of ALCAM to rolling, adhesion, and diapedesis of human CD14(+) monocytes across the human BBB under flow and static conditions. Taken together, our study highlights the potential differences in the CNS expression of ALCAM in mouse and human and supports a prominent role for ALCAM in the multi-step extravasation of monocytes across the BBB.
Faculties and Departments:03 Faculty of Medicine
03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Neurobiology (Schaeren-Wiemers)
UniBasel Contributors:Schaeren-Wiemers, Nicole and Zeis, Thomas
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:03 Aug 2020 11:49
Deposited On:03 Aug 2020 11:49

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