A resource for the conditional ablation of microRNAs in the mouse

Park, Chong Yon and Jeker, Lukas T. and Carver-Moore, Karen and Oh, Alyssia and Liu, Huey Jiin and Cameron, Rachel and Richards, Hunter and Li, Zhongmei and Adler, David and Yoshinaga, Yuko and Martinez, Maria and Nefadov, Michael and Abbas, Abul K. and Weiss, Art and Lanier, Lewis L. and de Jong, Pieter J. and Bluestone, Jeffrey A. and Srivastava, Deepak and McManus, Michael T.. (2012) A resource for the conditional ablation of microRNAs in the mouse. Cell Reports , 1 (4). pp. 385-391.

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Official URL: https://edoc.unibas.ch/61962/

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The importance of miRNAs during development and disease processes is well established. However, most studies have been done in cells or with patient tissues, and therefore the physiological roles of miRNAs are not well understood. To unravel in vivo functions of miRNAs, we have generated conditional, reporter-tagged knockout-first mice for numerous evolutionarily conserved miRNAs. Here, we report the generation of 162 miRNA targeting vectors, 64 targeted ES cell lines, and 46 germline-transmitted miRNA knockout mice. In vivo lacZ reporter analysis in 18 lines revealed highly tissue-specific expression patterns and their miRNA expression profiling matched closely with published expression data. Most miRNA knockout mice tested were viable, supporting a mechanism by which miRNAs act redundantly with other miRNAs or other pathways. These data and collection of resources will be of value for the in vivo dissection of miRNA functions in mouse models.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Molecular Immune Regulation (Jeker)
UniBasel Contributors:Jeker, Lukas T.
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:16 Dec 2020 07:59
Deposited On:16 Dec 2020 07:59

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