Effect of Immunosuppression on T-Helper 2 and B-Cell Responses to Influenza Vaccination

Egli, Adrian and Humar, Atul and Widmer, Lukas A. and Lisboa, Luiz F. and Santer, Deanna M. and Mueller, Thomas and Stelling, Joerg and Baluch, Aliyah and O'Shea, Daire and Houghton, Michael and Kumar, Deepali. (2015) Effect of Immunosuppression on T-Helper 2 and B-Cell Responses to Influenza Vaccination. The Journal of Infectious Diseases, 212 (1). pp. 137-146.

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Official URL: https://edoc.unibas.ch/61715/

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BACKGROUND: Influenza vaccine immunogenicity is suboptimal in immunocompromised patients. However, there are limited data on the interplay of T- and B- cell responses to vaccination with simultaneous immunosuppression. METHODS: We collected peripheral blood mononuclear cells from transplant recipients before and 1 month after seasonal influenza vaccination. Before and after vaccination, H1N1-specific T- and B-cell activation were quantified with flow cytometry. We also developed a mathematical model using T- and B-cell markers and mycophenolate mofetil (MMF) dosage. RESULTS: In the 47 patients analyzed, seroconversion to H1N1 antigen was demonstrated in 34%. H1N1-specific interleukin 4 (IL-4)-producing CD4(+) T-cell frequencies increased significantly after vaccination in 53% of patients. Prevaccine expression of H1N1-induced HLA-DR and CD86 on B cells was high in patients who seroconverted. Seroconversion against H1N1 was strongly associated with HLA-DR expression on B cells, which was dependent on the increase between prevaccine and postvaccine H1N1-specific IL-4(+)CD4(+) T cells (R(2) = 0.35). High doses of MMF (</= 2 g/d) led to lower seroconversion rates, smaller increase in H1N1-specific IL-4(+)CD4(+) T cells, and reduced HLA-DR expression on B cells. The mathematical model incorporating a MMF-inhibited positive feedback loop between H1N1-specific IL-4(+)CD4(+) T cells and HLA-DR expression on B cells captured seroconversion with high specificity. CONCLUSIONS: Seroconversion is associated with influenza-specific T-helper 2 and B-cell activation and seems to be modulated by MMF.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Applied Microbiology Research (Egli)
UniBasel Contributors:Egli, Adrian
Item Type:Article, refereed
Article Subtype:Research Article
ISSN:1537-6613 (Electronic) 0022-1899 (Linking)
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:20 Oct 2018 09:37
Deposited On:20 Oct 2018 09:37

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