A semisynthetic carbohydrate-lipid vaccine that protects against S. pneumoniae in mice

Cavallari, Marco and Stallforth, Pierre and Kalinichenko, Artem and Rathwell, Dominea C. K. and Gronewold, Thomas M. A. and Adibekian, Alexander and Mori, Lucia and Landmann, Regine and Seeberger, Peter H. and De Libero, Gennaro. (2014) A semisynthetic carbohydrate-lipid vaccine that protects against S. pneumoniae in mice. Nature Chemical Biology, 10 (11). pp. 950-956.

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Official URL: https://edoc.unibas.ch/61660/

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Severe forms of pneumococcal meningitis, bacteraemia and pneumonia result in more than 1 million deaths each year despite the widespread introduction of carbohydrate-protein conjugate vaccines against Streptococcus pneumoniae. Here we describe a new and highly efficient antipneumococcal vaccine design based on synthetic conjugation of S. pneumoniae capsule polysaccharides to the potent lipid antigen alpha-galactosylceramide, which stimulates invariant natural killer T (iNKT) cells when presented by the nonpolymorphic antigen-presenting molecule CD1d. Mice injected with the new lipid-carbohydrate conjugate vaccine produced high-affinity IgG antibodies specific for pneumococcal polysaccharides. Vaccination stimulated germinal center formation; accumulation of iNKT cells with a T follicular helper cell phenotype; and increased frequency of carbohydrate-specific, long-lived memory B cells and plasmablasts. This new lipid-carbohydrate vaccination strategy induced potent antipolysaccharide immunity that protected against pneumococcal disease in mice and may also prove effective for the design of carbohydrate-based vaccines against other major bacterial pathogens.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Experimental Immunology (De Libero)
UniBasel Contributors:De Libero, Gennaro
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Publishing Group
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:28 Jul 2020 13:40
Deposited On:21 Jul 2020 12:32

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