GABAB receptor cell-surface export is controlled by an endoplasmic reticulum gatekeeper

Doly, S. and Shirvani, H. and Gata, G. and Meye, F. J. and Emerit, M. B. and Enslen, H. and Achour, L. and Pardo-Lopez, L. and Yang, S. K. and Armand, V. and Gardette, R. and Giros, B. and Gassmann, M. and Bettler, B. and Mameli, M. and Darmon, M. and Marullo, S.. (2016) GABAB receptor cell-surface export is controlled by an endoplasmic reticulum gatekeeper. Molecular Psychiatry, 21 (4). pp. 480-490.

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Official URL: https://edoc.unibas.ch/61395/

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Endoplasmic reticulum (ER) release and cell-surface export of many G protein-coupled receptors (GPCRs) are tightly regulated. For gamma-aminobutyric acid (GABA)B receptors of GABA, the major mammalian inhibitory neurotransmitter, the ligand-binding GB1 subunit is maintained in the ER by unknown mechanisms in the absence of hetero-dimerization with the GB2 subunit. We report that GB1 retention is regulated by a specific gatekeeper, PRAF2. This ER resident transmembrane protein binds to GB1, preventing its progression in the biosynthetic pathway. GB1 release occurs upon competitive displacement from PRAF2 by GB2. PRAF2 concentration, relative to that of GB1 and GB2, tightly controls cell-surface receptor density and controls GABAB function in neurons. Experimental perturbation of PRAF2 levels in vivo caused marked hyperactivity disorders in mice. These data reveal an unanticipated major impact of specific ER gatekeepers on GPCR function and identify PRAF2 as a new molecular target with therapeutic potential for psychiatric and neurological diseases involving GABAB function.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Division of Physiology > Molecular Neurobiology Synaptic Plasticity (Bettler)
UniBasel Contributors:Bettler, Bernhard
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:20 Jan 2019 18:35
Deposited On:20 Jan 2019 18:35

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