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Indoor bacteria and asthma in adults : a multicentre case-control study within ECRHS II

Pekkanen, Juha and Valkonen, Maria and Täubel, Martin and Tischer, Christina and Leppänen, Hanna and Kärkkäinen, Päivi M. and Rintala, Helena and Zock, Jan-Paul and Casas, Lidia and Probst-Hensch, Nicole and Forsberg, Bertil and Holm, Mathias and Janson, Christer and Pin, Isabelle and Gislason, Thorarinn and Jarvis, Debbie and Heinrich, Joachim and Hyvärinen, Anne. (2018) Indoor bacteria and asthma in adults : a multicentre case-control study within ECRHS II. The European respiratory journal, 51 (2). p. 1701241.

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Official URL: https://edoc.unibas.ch/61183/

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Abstract

Both protective and adverse effects of indoor microbial exposure on asthma have been reported, but mostly in children. To date, no study in adults has used non-targeted methods for detection of indoor bacteria followed by quantitative confirmation.A cross-sectional study of 198 asthmatic and 199 controls was conducted within the European Community Respiratory Health Survey (ECRHS) II. DNA was extracted from mattress dust for bacterial analysis using denaturing gradient gel electrophoresis (DGGE). Selected bands were sequenced and associations with asthma confirmed with four quantitative PCR (qPCR) assays.15 out of 37 bands detected with DGGE, which had at least a suggestive association (p<0.25) with asthma, were sequenced. Of the four targeted qPCRs,; Clostridium; cluster XI confirmed the protective association with asthma. The association was dose dependent (aOR 0.43 (95% CI 0.22-0.84) for the fourth; versus; first quartile, p for trend 0.009) and independent of other microbial markers. Few significant associations were observed for the three other qPCRs used.In this large international study, the level of; Clostridium; cluster XI was independently associated with a lower risk of prevalent asthma. Results suggest the importance of environmental bacteria also in adult asthma, but need to be confirmed in future studies.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Epidemiology and Public Health (EPH) > Chronic Disease Epidemiology > Genetic Epidemiology of Non-Communicable Diseases (Probst-Hensch)
03 Faculty of Medicine > Departement Public Health > Sozial- und Präventivmedizin > Genetic Epidemiology of Non-Communicable Diseases (Probst-Hensch)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
UniBasel Contributors:Probst-Hensch, Nicole
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Munksgaard
ISSN:0903-1936
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:02 Jul 2018 08:26
Deposited On:02 Jul 2018 08:26

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