Protective anti-V antibodies inhibit Pseudomonas and Yersinia translocon assembly within host membranes

Goure, J. and Broz, P. and Attree, O. and Cornelis, G. R. and Attree, I.. (2005) Protective anti-V antibodies inhibit Pseudomonas and Yersinia translocon assembly within host membranes. Journal of infectious diseases, Vol. 192, H. 2. pp. 218-225.

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Official URL: http://edoc.unibas.ch/dok/A5259141

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Pathogenic Yersinia species and Pseudomonas aeruginosa share a similar type III secretion/translocation system. The translocation system consists of 3 secreted proteins, YopB/PopB, YopD/PopD, and LcrV/PcrV; the latter is known to be a protective antigen. In an in vitro assay, the translocation system causes the lysis of erythrocytes infected with wild-type (wt) P. aeruginosa. wt Y. enterocolitica is not hemolytic, but a multiknockout mutant deprived of all the effectors and of YopN ( Delta HOPEMN) is hemolytic. In the presence of antibodies against PcrV and Y. pestis LcrV, the hemolytic activity of P. aeruginosa was inhibited. Similarly, the hemolytic activity of Delta HOPEMN was inhibited in the presence of anti-LcrV antibodies. The assembly of the translocon, composed of PopB/D and YopB/D proteins, was disturbed in immunoprotected erythrocyte membranes, mimicking the phenotypes of V knockout mutants. Thus, protective antibodies against the V antigens of Yersinia species and P. aeruginosa act at the level of the formation of the translocon pore in membranes of infected host cells by blocking the function of LcrV/PcrV. The hemolysis assay could be adapted for high-throughput screening of anti-infectious compounds that specifically target the type III translocon.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology
05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Molecular Microbiology (Cornelis)
UniBasel Contributors:Broz, Petr and Cornelis, Guy R.
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Oxford University Press
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:01 Feb 2013 08:44
Deposited On:22 Mar 2012 13:32

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