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New insights into the karyotype evolution of the free-living flatworm; Macrostomum lignano; (Platyhelminthes, Turbellaria)

Zadesenets, Kira S. and Schärer, Lukas and Rubtsov, Nikolay B.. (2017) New insights into the karyotype evolution of the free-living flatworm; Macrostomum lignano; (Platyhelminthes, Turbellaria). Scientific reports, 7. p. 6066.

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Official URL: https://edoc.unibas.ch/59444/

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Abstract

The free-living flatworm; Macrostomum lignano; is a model organism for evolutionary and developmental biology studies. Recently, an unusual karyotypic diversity was revealed in this species. Specifically, worms are either ‘normal’ 2n = 8, or they are aneuploid with one or two additional large chromosome(s) (i.e. 2n = 9 or 2n = 10, respectively). Aneuploid worms did not show visible behavioral or morphological abnormalities and were successful in reproduction. In this study, we generated microdissected DNA probes from chromosome 1 (further called MLI1), chromosome 2 (MLI2), and a pair of similar-sized smaller chromosomes (MLI3, MLI4). FISH using these probes revealed that MLI1 consists of contiguous regions homologous to MLI2-MLI4, suggesting that MLI1 arose due to the whole genome duplication and subsequent fusion of one full chromosome set into one large metacentric chromosome. Therefore, one presumably full haploid genome was packed into MLI1, leading to hidden tetraploidy in the; M. lignano; genome. The study of; Macrostomum; sp. 8 — a sibling species of; M. lignano; — revealed that it usually has one additional pair of large chromosomes (2n = 10) showing a high homology to MLI1, thus suggesting hidden hexaploidy in its genome. Possible evolutionary scenarios for the emergence of the; M. lignano; and; Macrostomum; sp. 8 genomes are discussed.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Integrative Biologie > Evolutionary Biology (Schärer)
UniBasel Contributors:Schärer, Lukas
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Publishing Group
ISSN:2045-2322
e-ISSN:2045-2322
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:07 Sep 2020 08:43
Deposited On:07 Sep 2020 08:43

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