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Impact of Methods on the Measurement of mRNA Turnover

Wada, Takeo and Becskei, Attila. (2017) Impact of Methods on the Measurement of mRNA Turnover. International Journal of Molecular Sciences, 18 (12). p. 12.

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Abstract

The turnover of the RNA molecules is determined by the rates of transcription and RNA degradation. Several methods have been developed to study RNA turnover since the beginnings of molecular biology. Here we summarize the main methods to measure RNA half-life: transcription inhibition, gene control, and metabolic labelling. These methods were used to detect the cellular activity of the mRNAs degradation machinery, including the exo-ribonuclease Xrn1 and the exosome. On the other hand, the study of the differential stability of mature RNAs has been hampered by the fact that different methods have often yielded inconsistent results. Recent advances in the systematic comparison of different method variants in yeast have permitted the identification of the least invasive methodologies that reflect half-lives the most faithfully, which is expected to open the way for a consistent quantitative analysis of the determinants of mRNA stability.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Computational & Systems Biology > Synthetic Microbiology (Becskei)
UniBasel Contributors:Becskei, Attila and Wada, Takeo
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Molecular Diversity Preservation International
ISSN:1661-6596
e-ISSN:1422-0067
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:02 Feb 2018 13:30
Deposited On:02 Feb 2018 13:30

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