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Gene Transfer Agent Promotes Evolvability within the Fittest Subpopulation of a Bacterial Pathogen

Québatte, Maxime and Christen, Matthias and Harms, Alexander and Körner, Jonas and Christen, Beat and Dehio, Christoph. (2017) Gene Transfer Agent Promotes Evolvability within the Fittest Subpopulation of a Bacterial Pathogen. Cell Systems, 4 (6). 611-621.e6.

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Abstract

The Bartonella gene transfer agent (BaGTA) is an archetypical example for domestication of a phage-derived element to permit high-frequency genetic exchange in bacterial populations. Here we used multiplexed transposon sequencing (TnSeq) and single-cell reporters to globally define the core components and transfer dynamics of BaGTA. Our systems-level analysis has identified inner- and outer-circle components of the BaGTA system, including 55 regulatory components, as well as an additional 74 and 107 components mediating donor transfer and recipient uptake functions. We show that the stringent response signal guanosine-tetraphosphate (ppGpp) restricts BaGTA induction to a subset of fast-growing cells, whereas BaGTA particle uptake depends on a functional Tol-Pal trans-envelope complex that mediates outer-membrane invagination upon cell division. Our findings suggest that Bartonella evolved an efficient strategy to promote genetic exchange within the fittest subpopulation while disfavoring exchange of deleterious genetic information, thereby facilitating genome integrity and rapid host adaptation.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Molecular Microbiology (Dehio)
UniBasel Contributors:Dehio, Christoph
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Elsevier
ISSN:2405-4712
e-ISSN:2405-4720
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:04 Jul 2018 12:37
Deposited On:04 Jul 2018 12:37

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