Schmid, Nadine and Suppiger, Angela and Steiner, Elisabeth and Pessi, Gabriella and Kaever, Volkhard and Fazli, Mustafa and Tolker-Nielsen, Tim and Jenal, Urs and Eberl, Leo. (2017) High intracellular c-di-GMP levels antagonize quorum sensing and virulence gene expression in Burkholderia cenocepacia H111. Microbiology, 163 (5). pp. 754-764.
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Official URL: https://edoc.unibas.ch/59165/
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Abstract
The opportunistic human pathogen Burkholderia cenocepacia H111 uses two chemically distinct signal molecules for controlling gene expression in a cell density-dependent manner: N-acyl-homoserine lactones (AHLs) and cis-2-dodecenoic acid (BDSF). Binding of BDSF to its cognate receptor RpfR lowers the intracellular c-di-GMP level, which in turn leads to differential expression of target genes. In this study we analysed the transcriptional profile of B. cenocepacia H111 upon artificially altering the cellular c-di-GMP level. One hundred and eleven genes were shown to be differentially expressed, 96 of which were downregulated at a high c-di-GMP concentration. Our analysis revealed that the BDSF, AHL and c-di-GMP regulons overlap for the regulation of 24 genes and that a high c-di-GMP level suppresses expression of AHL-regulated genes. Phenotypic analyses confirmed changes in the expression of virulence factors, the production of AHL signal molecules and the biosynthesis of different biofilm matrix components upon altered c-di-GMP levels. We also demonstrate that the intracellular c-di-GMP level determines the virulence of B. cenocepacia to Caenorhabditis elegans and Galleria mellonella.
Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Growth & Development 05 Faculty of Science > Departement Biozentrum > Infection Biology > Molecular Microbiology (Jenal) 05 Faculty of Science > Departement Biozentrum > Growth & Development > Molecular Microbiology (Jenal) 05 Faculty of Science > Departement Biozentrum > Infection Biology |
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UniBasel Contributors: | Jenal, Urs |
Item Type: | Article, refereed |
Article Subtype: | Research Article |
Publisher: | Microbiology Society |
ISSN: | 1350-0872 |
e-ISSN: | 1465-2080 |
Note: | Publication type according to Uni Basel Research Database: Journal article |
Identification Number: |
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Last Modified: | 04 Jul 2018 12:33 |
Deposited On: | 04 Jul 2018 12:33 |
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