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Streptavidin as a Scaffold for Light-Induced Long-Lived Charge Separation

Keller, Sascha G. and Pannwitz, Andrea and Mallin, Hendrik and Wenger, Oliver S. and Ward, Thomas R.. (2017) Streptavidin as a Scaffold for Light-Induced Long-Lived Charge Separation. Chemistry - A European Journal, 23 (71). pp. 18019-18024.

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Official URL: http://edoc.unibas.ch/58966/

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Abstract

Long-lived photo-driven charge separation is demonstrated by assembling a triad on a protein scaffold. For this purpose, a biotinylated triarylamine was added to a Ru II –streptavidin conjugate bearing a methyl viologen electron acceptor covalently linked to the N -terminus of streptavidin. To improve the rate and lifetime of the electron transfer, a negative patch consisting of up to three additional negatively charged amino acids was engineered through mutagenesis close to the biotin-binding pocket of streptavidin. Time-resolved laser spectroscopy revealed that the covalent attachment and the negative patch were beneficial for charge separation within the streptavidin hosted triad; the charge separated state was generated within the duration of the excitation laser pulse, and lifetimes up to 3120 ns could be achieved with the optimized supramolecular triad.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Bioanorganische Chemie (Ward)
UniBasel Contributors:Ward, Thomas R. R. and Keller, Sascha and Pannwitz, Andrea and Mallin, Hendrik and Wenger, Oliver
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Wiley
ISSN:0947-6539
e-ISSN:1521-3765
Note:Publication type according to Uni Basel Research Database: Journal article -- The final publication is available at Wiley, see DOI link
Language:English
Identification Number:
Last Modified:04 Jul 2019 10:59
Deposited On:22 Jan 2018 13:04

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