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Microbial methanogenesis in the sulfate-reducing zone of sediments in the Eckernf`orde Bay, SW Baltic Sea

Maltby, Johanna and Steinle, Lea and Löscher, Carolin R. and Bange, Hermann W. and Fischer, Martin A. and Schmidt, Mark and Treude, Tina. (2018) Microbial methanogenesis in the sulfate-reducing zone of sediments in the Eckernf`orde Bay, SW Baltic Sea. Biogeosciences, 15 (1). pp. 137-157.

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Abstract

Benthic microbial methanogenesis is a known source of methane in marine systems. In most sediments, the majority of methanogenesis is located below the sulfatereducing zone, as sulfate reducers outcompete methanogens for the major substrates hydrogen and acetate. The coexistence of methanogenesis and sulfate reduction has been shown before and is possible through the usage of noncompetitive substrates by methanogens such as methanol or methylated amines. However, knowledge about the magnitude, seasonality, and environmental controls of this noncompetitive methane production is sparse. In the present study, the presence of methanogenesis within the sulfate reduction zone (SRZ methanogenesis) was investigated in sediments (0–30 cm below seafloor, cm b.s.f.) of the seasonally hypoxic Eckernförde Bay in the southwestern Baltic Sea. Water column parameters such as oxygen, temperature, and salinity together with porewater geochemistry and benthic methanogenesis rates were determined in the sampling area “Boknis Eck” quarterly from March 2013 to September 2014 to investigate the effect of seasonal environmental changes on the rate and distribution of SRZ methanogenesis, to estimate its potential contribution to benthic methane emissions, and to identify the potential methanogenic groups responsible for SRZ methanogenesis. The metabolic pathway of methanogenesis in the presence or absence of sulfate reducers, which after the addition of a noncompetitive substrate was studied in four experimental setups: (1) unaltered sediment batch incubations (net methanogenesis), (2) 14C-bicarbonate labeling experiments (hydrogenotrophic methanogenesis), (3) manipulated experiments with the addition of either molybdate (sulfate reducer inhibitor), 2- bromoethanesulfonate (methanogen inhibitor), or methanol (noncompetitive substrate, potential methanogenesis), and (4) the addition of 13C-labeled methanol (potential methylotrophic methanogenesis). After incubation with methanol, molecular analyses were conducted to identify key functional methanogenic groups during methylotrophic methanogenesis. To also compare the magnitudes of SRZ methanogenesis with methanogenesis below the sulfate reduction zone (> 30 cm b.s.f.), hydrogenotrophic methanogenesis was determined by 14C-bicarbonate radiotracer incubation in samples collected in September 2013.
Faculties and Departments:05 Faculty of Science
05 Faculty of Science > Departement Umweltwissenschaften
05 Faculty of Science > Departement Umweltwissenschaften > Umweltgeowissenschaften
05 Faculty of Science > Departement Umweltwissenschaften > Umweltgeowissenschaften > Geochemie Stoffkreisläufe (Lehmann)
UniBasel Contributors:Steinle, Lea
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:European Geosciences Union
ISSN:1726-4170
e-ISSN:1726-4189
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:18 Jun 2018 11:32
Deposited On:18 Jun 2018 11:30

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