Context-dependent deposition and regulation of mRNAs in P-bodies

Wang, Congwei and Schmich, Fabian and Srivatsa, Sumana and Weidner, Julie and Beerenwinkel, Niko and Spang, Anne. (2018) Context-dependent deposition and regulation of mRNAs in P-bodies. eLife, 7. e29815.

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Cells respond to stress by remodeling their transcriptome through transcription and degradation. Xrn1p-dependent degradation in P-bodies is the most prevalent decay pathway, yet, P-bodies may facilitate not only decay, but also act as a storage compartment. However, which and how mRNAs are selected into different degradation pathways and what determines the fate of any given mRNA in P-bodies remain largely unknown. We devised a new method to identify both common and stress-specific mRNA subsets associated with P-bodies. mRNAs targeted for degradation to P-bodies, decayed with different kinetics. Moreover, the localization of a specific set of mRNAs to P-bodies under glucose deprivation was obligatory to prevent decay. Depending on its client mRNA, the RNA-binding protein Puf5p either promoted or inhibited decay. Furthermore, the Puf5p-dependent storage of a subset of mRNAs in P-bodies under glucose starvation may be beneficial with respect to chronological lifespan.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Biochemistry (Spang)
UniBasel Contributors:Spang, Anne
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:eLife Sciences Publications
Note:Publication type according to Uni Basel Research Database: Journal article -- Additional publication or translation in: https://www.biorxiv.org/content/early/2017/07/04/159376
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Last Modified:27 Apr 2018 14:27
Deposited On:12 Jan 2018 07:07

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