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Mutation of Conserved Residues Increases in Vitro Activity of the Formylglycine-Generating Enzyme

Knop, Matthias and Lemnaru, Roxana and Seebeck, Florian P.. (2017) Mutation of Conserved Residues Increases in Vitro Activity of the Formylglycine-Generating Enzyme. ChemBioChem, 18 (17). pp. 1755-1761.

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Official URL: http://edoc.unibas.ch/58308/

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Abstract

The formylglycine-generating enzyme (FGE) recognizes proteins with a specific cysteine-containing six-amino-acid motif and converts this cysteine residue into formylglycine. The resulting aldehyde function provides a unique handle for selective protein labeling. We have identified two mutations in FGE from Thermomonospora curvata that increase this catalytic efficiency more than 40-fold. The resulting activity and stability, as well as its ease of recombinant production, make this FGE variant a practical reagent for in vitro protein engineering.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Molecular Bionics (Seebeck)
UniBasel Contributors:Seebeck, Florian Peter
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Wiley
ISSN:1439-4227
e-ISSN:1439-7633
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:05 Jan 2018 13:46
Deposited On:05 Jan 2018 13:46

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