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Incomplete recovery of intact polar glycerol dialkyl glycerol tetraethers from lacustrine suspended biomass

Weber, Yuki and Sinninghe Damsté, Jaap S. and Hopmanns, Ellen C. and Lehmann, Moritz F. and Niemann, Helge. (2017) Incomplete recovery of intact polar glycerol dialkyl glycerol tetraethers from lacustrine suspended biomass. Limnology and Oceanography: Methods, 15 (9). pp. 782-793.

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Official URL: http://edoc.unibas.ch/57951/

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Abstract

Branched and isoprenoid glycerol dialkyl glycerol tetraethers (GDGTs) are membrane lipids of bacteria and archaea, respectively, and their core lipid distributions are used as proxy indicators in paleolimnological studies. In addition, the amount and composition of intact polar lipid (IPL) GDGTs yield information on the presence and abundance of GDGT-producing microbes within the water column. GDGTs are, however, not always easily recovered from cultured microbial cells by commonly applied extraction methods, and this may also apply to suspended particulate matter (SPM) in aquatic systems. In order to investigate potential biases induced by incomplete GDGT recovery, we analyzed both core- and IPL-GDGTs in SPM from Lake Lugano (Switzerland) using (1) ultrasonic solvent extraction with mixtures of methanol (MeOH) and dichloromethane (DCM), and (2) two modifications of the Bligh–Dyer (BD) protocol. Acid hydrolysis of the post-extraction residues revealed that particularly branched GDGTs were poorly recovered from SPM by the MeOH/DCM mixtures (25–62%). Much better extraction yields (> 85%) were achieved with the BD method, however, during subsequent phase separation, up to 75% of the extracted branched IPL-GDGTs partitioned into the aqueous phosphate buffer that is usually discarded. In contrast, when this buffer was substituted with 5% trichloroacetic acid, only 13% were lost into the aqueous phase. Depending on the protocol used, the distribution of the IPL-derived GDGTs varied substantially. Our results indicate that both bacterial- and archaeal IPL-GDGTs can be difficult to extract from lacustrine microbial communities, and caution is advised when targeting the “viable” GDGT pool.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Geowissenschaften > Geochemie Stoffkreisläufe (Lehmann)
UniBasel Contributors:Lehmann, Moritz F and Niemann, Helge and Weber, Yuki
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Wiley
ISSN:1541-5856
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:09 Sep 2019 13:36
Deposited On:28 May 2018 15:28

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