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Cyclic di-GMP differentially tunes a bacterial flagellar motor through a novel class of CheY-like regulators

Nesper, Jutta and Hug, Isabelle and Kato, Setsu and Hee, Chee-Seng and Habazettl, Judith Maria and Manfredi, Pablo and Grzesiek, Stephan and Schirmer, Tilman and Emonet, Thierry and Jenal, Urs. (2017) Cyclic di-GMP differentially tunes a bacterial flagellar motor through a novel class of CheY-like regulators. eLife, 6. e28842.

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Official URL: http://edoc.unibas.ch/57179/

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Abstract

The flagellar motor is a sophisticated rotary machine facilitating locomotion and signal transduction. Owing to its important role in bacterial behavior, its assembly and activity are tightly regulated. For example, chemotaxis relies on a sensory pathway coupling chemical information to rotational bias of the motor through phosphorylation of the motor switch protein CheY. Using a chemical proteomics approach, we identified a novel family of CheY-like (Cle) proteins in Caulobacter crescentus, which tune flagellar activity in response to binding of the second messenger c-di-GMP to a C-terminal extension. In their c-di-GMP bound conformation Cle proteins interact with the flagellar switch to control motor activity. We show that individual Cle proteins have adopted discrete cellular functions by interfering with chemotaxis and by promoting rapid surface attachment of motile cells. This study broadens the regulatory versatility of bacterial motors and unfolds mechanisms that tie motor activity to mechanical cues and bacterial surface adaptation.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Schirmer)
UniBasel Contributors:Schirmer, Tilman and Nesper, Jutta and Hug, Isabelle and Hee, Chee Seng and Habazettl, Judith Maria and Manfredi, Pablo and Grzesiek, Stephan and Jenal, Urs
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:eLife Sciences Publications
ISSN:2050-084X
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
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Last Modified:17 Apr 2018 10:34
Deposited On:16 Apr 2018 14:15

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