Live imaging molecular changes in junctional tension upon VE-cadherin in zebrafish

Lagendijk, Anne Karine and Gomez, Guillermo A. and Baek, Sungmin and Hesselson, Daniel and Hughes, William E. and Paterson, Scott and Conway, Daniel E. and Belting, Heinz-Georg and Affolter, Markus and Smith, Kelly A. and Schwartz, Martin A. and Yap, Alpha S. and Hogan, Benjamin M.. (2017) Live imaging molecular changes in junctional tension upon VE-cadherin in zebrafish. Nature Communications, 8 (1). p. 1402.

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Forces play diverse roles in vascular development, homeostasis and disease. VE-cadherin at endothelial cell-cell junctions links the contractile acto-myosin cytoskeletons of adjacent cells, serving as a tension-transducer. To explore tensile changes across VE-cadherin in live zebrafish, we tailored an optical biosensor approach, originally established in vitro. We validate localization and function of a VE-cadherin tension sensor (TS) in vivo. Changes in tension across VE-cadherin observed using ratio-metric or lifetime FRET measurements reflect acto-myosin contractility within endothelial cells. Furthermore, we apply the TS to reveal biologically relevant changes in VE-cadherin tension that occur as the dorsal aorta matures and upon genetic and chemical perturbations during embryonic development.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Cell Biology (Affolter)
UniBasel Contributors:Affolter, Markus
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Publishing Group
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:16 May 2018 07:25
Deposited On:16 May 2018 07:25

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